The kink-turn: a new RNA secondary structure motif

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Plasticity of the RNA kink turn structural motif.

The kink turn (K-turn) is an RNA structural motif found in many biologically significant RNAs. While most examples of the K-turn have a similar fold, the crystal structure of the Azoarcus group I intron revealed a novel RNA conformation, a reverse kink turn bent in the direction opposite that of a consensus K-turn. The reverse K-turn is bent toward the major grooves rather than the minor groove...

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Biochemical characterization of the kink-turn RNA motif.

RNA, which acts as a medium for transmitting genetic information, plays a variety of roles in a cell. As with proteins, elucidation of the three- dimensional (3D) structures of RNAs is important for understanding their various roles. Determination of the atomic structures of crystallized ribosome has enabled the identification of previously unknown RNA structural motifs. The kink-turn (K-turn o...

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The Kink Turn, a Key Architectural Element in RNA Structure

Kink turns (k-turns) are widespread structural elements that introduce an axial bend into duplex RNA with an included angle of 50°. These mediate key tertiary interactions and bind specific proteins including members of the L7Ae family. The standard k-turn comprises a three-nucleotide bulge followed by G·A and A·G pairs. The RNA kinks by an association of the two minor grooves, stabilized by th...

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The λ-Turn: A New Structural Motif in Ribosomal RNA

RNA structural motifs are recurrent structural elements occurring in RNA molecules. They play essential roles in consolidating RNA tertiary structures and in binding proteins. Recently, we identified a new type of RNA structural motif, namely λ-turn, from ribosomal RNAs. This motif has a helix-internal loop-helix structure. The directions of its two helices are changed *90° due to the existence...

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RNA Tertiary Interactions in a Riboswitch Stabilize the Structure of a Kink Turn

The kink turn is a widespread RNA motif that introduces an acute kink into the axis of duplex RNA, typically comprising a bulge followed by a G⋅A and A⋅G pairs. The kinked conformation is stabilized by metal ions, or the binding of proteins including L7Ae. We now demonstrate a third mechanism for the stabilization of k-turn structure, involving tertiary interactions within a larger RNA structur...

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ژورنال

عنوان ژورنال: The EMBO Journal

سال: 2001

ISSN: 1460-2075

DOI: 10.1093/emboj/20.15.4214